FastQCFastQC Report
Wed 25 May 2016
SRR1780990_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780990_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247940
Sequences flagged as poor quality0
Sequence length76
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6913427.883358877147696No Hit
CTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTG18950.764297813987255No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG150.002221569770.01008622
TATGCGC150.002225128269.981853
GTGCCGG200.00693613952.51816618
CGGGTCC200.006941677552.5075732
TAAACGC200.006941677552.5075717
TTTGGCG200.0069472252.4969859
GACTGAC200.00695276552.486397
TCTGGCG800.052.4863911
AATCGTC305.866262E-446.67339328
TGATACT305.8779435E-446.6545644
GGACTGA305.8779435E-446.6545646
CGCGTCT551.3097088E-744.5339057
TGCCGGC602.5858935E-740.8392219
GCGCTGC953.6379788E-1240.53215815
TAATCGT350.001252975740.00576427
GATACAG350.001255464939.9896281
TGTGCGC350.001255464939.9896283
GCCGTAT3200.039.38067654
CGTCTGG801.4188117E-939.364799
TCGCCGT3300.039.2480852
Produced by FastQC (version 0.11.5)