FastQCFastQC Report
Wed 25 May 2016
SRR1780990_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780990_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247940
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT4794119.33572638541583No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG55352.2323949342582883No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32861.3253206420908283No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC25941.046220859885456No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT16610.6699201419698314RNA PCR Primer, Index 48 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC12530.5053642010163749No Hit
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC7730.31176897636524964No Hit
ATTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT6920.2790997822053723No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC4460.17988222957167058RNA PCR Primer, Index 48 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT3590.1447930951036541No Hit
CTTATACACATCTCCGAGCCCACGAGACTATCGTCAATCTCGTATGCCGT3510.14156650802613535No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG3480.1403565378720658No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC2760.11131725417439704No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGACGT2630.10607405017342905No Hit
TATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCT2580.10405743324997983RNA PCR Primer, Index 48 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTACA150.002220021370.022457
TGCCGTA207.9196514E-569.99415645
AACTCGT600.064.1613137
AGGGGGG56700.063.0194370
ACGTATG453.8380676E-1062.2170240
AAACTCG800.061.24488436
CGTATGA1050.056.6619341
AAGGGGG66450.053.6674569
CACGGGT200.006936853852.516826
GCCGGTA200.006936853852.516855
TCGGCTT200.006936853852.516824
TGCGCGA200.006936853852.516828
CCGGTAC200.006936853852.516856
ATATCGA200.006947933752.49561737
CTTGACA200.006947933752.49561763
CGACTTA200.006947933752.49561748
CTTGAAA71250.051.74286757
CTGCTTG69800.051.61392654
TTGAAAA71950.051.5800958
CGTCTTC70250.051.56152348