FastQCFastQC Report
Wed 25 May 2016
SRR1780989_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780989_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366905
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG332569.063926629509No Hit
CTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTG11040.3008953271282757No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA150.002225256869.987662
TGGAAAC200.006953155652.49069614
CCGGATG305.8634905E-446.68384628
GCCGGAT305.8634905E-446.68384627
TCGCCGT1950.043.06929452
GATCTCG2050.042.68118341
CGGTGGT1900.042.36091246
CTCGGTG2000.041.9925644
GCCGTAT2100.041.65928654
GTCGCCG2200.041.35630851
TCGGTGG2050.040.96835345
AGATCTC2150.040.69600740
ATCTCGG2200.039.771142
TGGTCGC2050.039.26133749
GTAGATC2400.036.4767338
TCTCGGT2550.035.67995543
TAGATCT2400.035.0081139
ACGACGA400.002417779534.99856621
CCGAGTT400.002419396834.99379759
GGTGGTC2600.033.64788447
Produced by FastQC (version 0.11.5)