FastQCFastQC Report
Wed 25 May 2016
SRR1780989_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780989_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences366905
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT197815.391313827830093No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG31580.8607132636513539No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20010.5453727804199998No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC11450.31206988184952505No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT10700.2916286232130933TruSeq Adapter, Index 2 (95% over 21bp)
ATTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT7630.20795573786129926No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC6130.16707322058843568No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTCGT401.3460522E-1070.0069537
AACTCGA150.002222818670.0069537
GCAGCGT401.3460522E-1069.997411
GACGACA150.002224020469.99740626
GCCGTAT150.002224020469.99740646
TGCCGTA150.002224020469.99740645
AATCTCG800.061.2560836
AGGGGGG27750.060.4204170
AAGCCGT352.971119E-759.99778444
TAACTCG650.059.23665236
ACGTATG452.6946509E-854.4498540
GCCGTCA1050.053.3313646
CTGGCTA200.006945555652.5052159
CGTAACT608.367351E-1152.5052134
CTCCTGG200.006949300452.498061
ACGTAAC608.367351E-1152.49805533
TATGCCG34250.050.7864443
GCCGTCT32950.050.7720246
CCGTCTT32900.050.74280547
CGAATCT900.050.56057434