FastQCFastQC Report
Wed 25 May 2016
SRR1780988_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780988_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275775
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3888214.099175052125828No Hit
CTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTG8180.296618620252017No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTA150.002225871769.9780651
GGTCACT200.00694012652.5121123
TTGTATC200.00695507952.4835472
TGCGCGT200.00695507952.4835475
ACCGTGT200.00695507952.4835478
GATCTTC200.00695507952.4835471
CCCACGT305.8596674E-446.685933
CACGTAA305.8596674E-446.685935
CGGTGGT1300.043.06342346
CTCGGTG1350.041.47644
TCGGTGG1350.041.46848345
AGATCTC1400.040.01648740
GACTATC350.001251569740.01648730
ACGTTAG350.001254924539.99471744
TCTCGGT1400.039.99471743
TGGTCGA350.001256044339.9874649
GTAGATC1500.039.68301838
GATCTCG1450.038.6366141
ATCTCGG1450.038.62959742
TCGCCGT1300.037.68049652
Produced by FastQC (version 0.11.5)