FastQCFastQC Report
Wed 25 May 2016
SRR1780988_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780988_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275775
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT253369.187199709908441No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG32701.1857492521076964No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15540.5635028555887952No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC14240.5163629770646361No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT11160.40467772640739735RNA PCR Primer, Index 47 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC7140.2589067174326897No Hit
ATTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT5290.1918230441483093No Hit
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC4290.15556159912972534No Hit
CTTTACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC3110.1127730940077962No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCAGA150.002221644670.011611
GGCATAT150.002221644670.011611
AGGGGGG31650.060.15704370
AGCCGTC352.967936E-759.99906545
CCGACTT308.364052E-658.33242847
ACGTATG308.364052E-658.33242840
CGGAATC200.00694688952.49918420
CCCTCGA200.00694688952.49918419
CAGCACG200.00694688952.49918444
CTTATGG200.00694688952.49918435
AGTGACG200.00694688952.49918417
GCGTCTC200.00694688952.49918417
CCGTTCC200.00694688952.49918459
GCCGTAT200.00694688952.49918446
GAGATCC200.00694688952.49918462
TGCCGAC407.464223E-752.49918445
CGTATGA900.050.55476841
GCCGTCT38700.050.1025846
CCGTCTT38450.050.0642447
CTTGAAA40250.049.99922657