FastQCFastQC Report
Wed 25 May 2016
SRR1780987_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780987_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334241
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6747620.187828542877746No Hit
CTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTG17570.5256686043902453No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATGG252.3878441E-456.0034681
TCGCAAA200.006941930452.51111623
GTTGTGC200.006946040352.5032541
CAGTAGT200.006946040352.5032541
GACTTGC200.00695015252.495415
ACTAGAT200.00695015252.49547
CTGGCAA200.00695015252.495414
AAGCTTG200.00695015252.495418
TCGCCGT3150.045.5583852
GTCGCCG3300.044.54821851
CGGTGGT3400.044.26082646
GCCGTAT3200.043.75271254
TGGTCGC3450.042.60496549
CGTATCA3300.042.42687256
CTCGGTG3500.041.9963244
TCGGTGG3500.041.9963245
GATCTCG3500.040.00248341
AAGCGCA350.001255031939.99649448
TCTCGGT3500.039.99649443
AGATCTC3450.039.56767340
Produced by FastQC (version 0.11.5)