FastQCFastQC Report
Wed 25 May 2016
SRR1780987_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780987_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334241
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT4620613.82415682097648No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG56221.682019859921434No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC34001.0172300824853924No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32070.9594873160384274No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT13810.41317492468009615Illumina PCR Primer Index 9 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC11600.34705496931854557No Hit
ATTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT7610.22768002728570103No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC6420.19207697439871232No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGCTCTCGTATGCCGT4340.12984642817607653No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACAGATCTCGTATGCCGT4290.12835050158418626No Hit
CTTTACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC3900.11668227416744205No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGATC500.069.9979133
CGTGCGG150.002223783969.997941
TCGTCGC150.002223783969.997912
CTCGAAA207.921579E-569.997939
AGGGGGG57000.063.4279370
TCGAAAG308.36863E-658.3315940
TCGAATG850.057.64533240
CGAATGC750.055.9983241
CTTTATA2200.054.1054762
CGACTTC653.6379788E-1253.8445448
AAGGGGG67200.053.7483969
GGGGTAC200.00694034852.514145
GTCTGGA200.00694445752.5062831
GGTCCCA200.00694445752.5062831
CTTGTCG200.00694856852.4984324
AACGTAT200.00694856852.4984339
TTGGACC200.00694856852.4984328
TGCCTTC407.469789E-752.4984345
CCGTCTT70700.051.83013547
TCTTCTG71300.051.73758350
Produced by FastQC (version 0.11.5)