FastQCFastQC Report
Wed 25 May 2016
SRR1780986_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780986_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207971
Sequences flagged as poor quality0
Sequence length76
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5649927.166768443677242No Hit
CTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTG14040.6750941236999389No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTTA150.00222303269.9944746
TCGATGC150.00222303269.9944740
TTAGTCG150.00222303269.9944768
CGTTACC200.006939638452.50847617
GGAACCG200.006939638452.50847659
AAACGTA200.00694624352.49585313
CATGTCC200.00694624352.4958534
GCGAAAA305.8705255E-446.6629850
TCTAGCA404.5839086E-543.7465443
TCTTTAT404.5839086E-543.7465441
TCGCCGT2650.043.58146352
GCCGTAT2650.043.58146354
CGTATCA2900.041.0411156
TGGTCGC2900.041.03124249
CCGTATC2850.040.52311755
GTCGCCG2950.040.33579651
TCGGTGG3050.040.1607645
CGCGGTA350.001252403440.0064658
GATCTCG2800.039.9968441
TCATTAA2900.039.8340260
Produced by FastQC (version 0.11.5)