FastQCFastQC Report
Wed 25 May 2016
SRR1780986_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780986_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207971
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT3837818.45353438700588No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG45202.173379942395815No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24121.1597770843050232No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC24101.1588154117641403No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT12810.615951262435628RNA PCR Primer, Index 42 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC10910.5245923710517332No Hit
ATTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT6700.3221603011958398No Hit
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC5540.2663832938246198RNA PCR Primer, Index 32 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC3750.18031360141558198No Hit
CTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG3340.16059931432747834No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGCTCTCGTATGCCGT2660.12790244793745284No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT2570.12357492150347886No Hit
CTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG2290.1101115059311154No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC2140.10289896187449211No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTGG207.914456E-569.9980834
CACGTAT252.833276E-669.9980839
ATCTCGA600.064.164937
AGGGGGG45700.063.56570
ACGTATG352.964607E-759.9983540
CGAATGC601.8189894E-1258.3317341
GCCGTCA950.055.2616446
AAGGGGG54300.053.23978869
GCCATAG200.006938223352.5111851
AGTCCGC200.006944826352.49855822
CGTGCCG200.006944826352.49855856
TCGATCA407.455874E-752.49855834
CGATCAC407.455874E-752.49855835
TCGAATG800.052.49855840
TGCCGTC58200.051.2958445
CTGCTTG57600.051.2833154
TTGAAAA58500.051.272158
CGTCTTC57800.051.22696348
ATGCCGT58250.051.19172744
CTTGAAA58450.051.13631457
Produced by FastQC (version 0.11.5)