FastQCFastQC Report
Wed 25 May 2016
SRR1780985_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780985_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences262742
Sequences flagged as poor quality0
Sequence length76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8363031.82970366367006No Hit
CTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTG21370.813345411087683No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTAG150.002225267769.981928
CGCGTCG308.3749965E-658.3182687
GCGCGTC553.6379788E-1157.257936
TGCGCGT553.6379788E-1157.257935
GGTCAGT200.006947964452.496431
GTGTCAC200.00695319852.486443
GGCAAGC305.8565254E-446.6901623
TGTGCGC551.3101089E-744.5339473
GACCTAG404.586923E-543.7470251
GCGTCGG404.5920817E-543.73878
CTTTATA503.472227E-641.9971472
GATCTCG4300.041.52461641
ATCTCGG4350.041.0395142
TCGGTGG4700.040.95466245
GCCGTAT4200.040.8305654
GTGCGCG602.5948611E-740.8227844
TCTCGGT4550.040.7742243
TCGCCGT4300.040.6949152
CTCGGTG4650.040.6501344
AGATCTC4400.040.57314740
Produced by FastQC (version 0.11.5)