FastQCFastQC Report
Wed 25 May 2016
SRR1780985_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780985_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences262742
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT5854822.28345677508735No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG68502.607120292910916No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38001.446285709935983No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC37391.4230690182764842No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT18680.71096360688432RNA PCR Primer, Index 15 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC15250.5804172914874668No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC8440.32122766820683407No Hit
ATTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT7580.2884959389819671No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGATAATCTCGTATGCCGT6370.24244315716558448No Hit
CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC4780.18192751824984205No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTAAATCTCGTATGCCGT4050.1541436085589666No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTATCTCGTATGCCGTC3670.13968075145960676No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT3670.13968075145960676No Hit
CTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG3500.1332105259151563No Hit
CTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG3410.1297851123916237No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGACGT3340.1271209018733206No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC3240.12331488684717327No Hit
TATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCT3000.1141804507844197No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGCG150.002221909670.00857567
TACCGTC150.002221909670.00857545
CGAATGC353.6943675E-970.0085741
CACGTAT207.912454E-570.0085739
ATACCGT207.912454E-570.0085744
TTCCGTT150.00222358869.9952511
CGCCACG150.00222358869.9952522
AACTCGT850.065.8904137
AGGGGGG69200.063.38203470
AGCGTCA401.0635631E-861.245843
ATGACGA252.3861874E-456.00685544
CCGACTT750.056.00685547
ACGTATG252.3861874E-456.00685540
AAGGGGG81850.053.58627769
CGACTTC850.053.5359648
TCTTTAT2450.052.8535461
AAACTCG1000.052.50642836
CGTATGA1600.052.50642441
TGCCGTA200.00694273652.50642446
ACTGACC200.006947965452.49643362
Produced by FastQC (version 0.11.5)