FastQCFastQC Report
Wed 25 May 2016
SRR1780984_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780984_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483086
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6403613.25561080221741No Hit
CTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTG14130.29249450408415895No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTAG150.002220196470.0311724
TGTGCGC150.002226588569.980443
GGCACGT200.00693453852.52882433
CGCTAGG200.006937379552.52338425
CCGGTTC200.006937379552.52338435
TCACGTG200.006957295452.4853317
GTGATAC350.001256772139.98882348
AGCGAGC350.001256772139.98882313
GCCGTAT3300.036.0505354
GTTAAGC708.698007E-734.9938461
TCGCCGT3400.034.9902252
TCGGTGG4050.032.8337345
CTCGGTG3950.032.7790444
CCGGTCT652.1055326E-532.2986649
GTCGCCG3700.032.15317551
TCAACCG552.9812873E-431.83565132
TCTCGGT4200.031.6643743
CGGTGGT4000.031.491246
TGGTCGC3800.031.3070449
GAATCCC450.00429881731.12174223
Produced by FastQC (version 0.11.5)