FastQCFastQC Report
Wed 25 May 2016
SRR1780984_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780984_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences483086
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT429508.890756511263005No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG53551.108498279809392No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25600.5299263485176553No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC24160.5001179914135371No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT15110.3127807471133504Illumina PCR Primer Index 9 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC11710.24239990395084932No Hit
ATTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT8160.1689140235900026No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC6390.1322745846495241No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTGC150.002223391370.005834
TGCCGAC550.063.63504445
AGGGGGG54950.061.91595570
GCCGACT601.8189894E-1258.33212746
AGATCGT252.3898897E-455.9988432
TGACGAC252.3898897E-455.9988445
ATATCGT800.052.5043537
TCGTATC200.006947333552.5043540
TCGCCGT200.00695017852.49891750
GTCGAGG200.00695017852.4989177
GGTCGAG200.00695017852.4989176
GCGAATC200.00695017852.49891725
TCGAGGG200.00695017852.4989178
CGCCGTC200.00695017852.49891751
GCGTCTA200.00695017852.49891714
CCCGAGG200.00695017852.49891758
CCGTCTT65500.052.25845747
GCCGTCT65950.052.16723346
CGTCTTC66550.052.01244748
TATGCCG66750.051.9090443