FastQCFastQC Report
Wed 25 May 2016
SRR1780983_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780983_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392432
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5480313.964967179027193No Hit
CTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTG11710.29839564561503645No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGT150.002219877470.03123532
GGTATCA252.3913477E-455.98931
GTCCCGC200.006943389352.51003620
GACGTAT200.00695389752.48996754
TGGTCGC2350.046.1613949
TCTCGGT2750.045.82110243
TCGGTGG2650.044.8970845
CTCGGTG2700.044.07126644
CGGTGGT2650.043.57657646
GCCGTAT2350.043.1832354
GATCTCG2700.042.78595741
ATCTCGG2750.042.0080342
GTAGATC2800.041.2578938
GGTGGTC3100.040.6373947
AGATCTC3100.039.52368540
TCGCCGT2500.039.19250552
GTACTAG459.201176E-538.8814541
GGTCTAA459.201176E-538.8814541
TAGATCT3100.038.39443639
GATCATA556.6967186E-638.174521
Produced by FastQC (version 0.11.5)