FastQCFastQC Report
Wed 25 May 2016
SRR1780983_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780983_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392432
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT343518.753363640070127No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG45761.1660618909772904No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC24020.6120805642761039No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22480.5728380967912912No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT10410.2652688873486362RNA PCR Primer, Index 30 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC8790.22398785012435274No Hit
ATTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT6290.16028254576589065No Hit
CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC5540.141170954458352No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCAGGATCTCGTATGCCGT4660.11874668732417336No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGACGT4420.11263097810576099No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTAGTATGCCGT4350.11084722958372406No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG42350.062.72704370
CGACTTC401.0648364E-861.2497848
TGCCGAC401.0648364E-861.2497845
AGCCGTC1500.058.33312245
CGGAACT252.3891329E-455.99979834
CGGATAT1100.054.09071434
CGTCTTA2400.053.95813448
ACTGCCG200.00694874252.4998137
GGATATC1000.052.49981335
CGCGACA200.00694874252.49981370
GCCGAAA200.00694874252.49981346
CGCTAGG200.00694874252.49981322
ACGACTA200.00694874252.49981347
AAGCCGT1750.051.99981344
CCGTCTT49550.051.5638947
GCCGTCT49600.051.5119146
CGTCTTC48600.051.41956348
TCTTCTG45800.051.04784850
ATATCGT1100.050.90890537
CCGACTT552.2810127E-950.90890547
Produced by FastQC (version 0.11.5)