FastQCFastQC Report
Wed 25 May 2016
SRR1780982_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780982_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences751973
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8293811.029385363570235No Hit
CTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTG64130.852823173172441No Hit
ATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTGGTC9310.1238076367103606No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGTA150.002225417269.9935461
CGTGTCA200.00694632952.5091328
AGCGTAG200.00695364152.495162
CTCGGTG11150.048.3363544
AGATCTC11350.048.10449240
TCGCCGT10950.047.94716352
GCCGTAT11100.047.92988654
TCGGTGG11350.047.79295345
GATCTCG11450.047.68436441
TGGTCGC11250.047.5987849
ATCTCGG11400.047.58650242
CGGTGGT11350.047.4846146
GTAGATC11800.046.86943438
GTCGCCG11350.046.25739751
TCTCGGT11850.045.7794243
TAGATCT12250.045.14470339
CGCCGTA12200.043.60833753
GGTGGTC13100.041.6755547
CGTATCA12850.041.39971556
GTATCAT13400.040.22017757
Produced by FastQC (version 0.11.5)