FastQCFastQC Report
Wed 25 May 2016
SRR1780982_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780982_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences751973
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT584417.771688611160241No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG109101.4508499640279637No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG65900.8763612523321982No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC42970.571430091239978No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT18070.2403011810264464Illumina PCR Primer Index 10 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC14070.18710778179535703No Hit
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC7520.1000035905544481Illumina PCR Primer Index 10 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTTA207.9262754E-570.00155
CCAATTT150.002225065669.9963416
TAACCGA150.002225065669.9963411
AGGGGGG72250.061.86247370
GTATTCT601.8189894E-1258.33804349
TGCCGAC800.056.87581345
GATTCCA252.3903359E-456.0007971
GCAGCGT653.6379788E-1253.846921
CTTTATA1900.053.4182622
CGTCTTC84500.053.27057348
CCGTCTT84000.053.0042847
GCCGTCT84300.052.98173546
TATGCCG87300.052.92171543
TCTTCTG83550.052.86730250
CTCGTAT89000.052.81887439
CGTATGC88050.052.7491941
TCGTATG89500.052.68023340
CTGCTTG83850.052.55292554
TCTCGTA89650.052.51399638
ACGTATG407.4767013E-752.50424240