FastQCFastQC Report
Wed 25 May 2016
SRR1780981_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780981_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362892
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7078019.504425559119518No Hit
CTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTG17680.4871972928584813No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG150.002222128770.0122725
ATACGCG150.002225776269.983324
GGACGGG200.006947193352.50196522
GGTCCGA200.006950981452.4947281
GACTTAT200.006950981452.4947281
GGGACGT200.006950981452.4947281
GGTCTAA200.006950981452.4947281
GAGAGCG200.006954771552.4874958
TACGCGT200.006954771552.4874955
TATACGC407.482322E-752.4874953
ACGCGTC200.006954771552.4874956
TGCGGGT352.0875657E-549.988095
TCGGTGG3850.049.9880945
ATCTCGG3200.049.22059242
GCCGTAT3350.049.10631654
CGGTGGT3800.048.80416546
TGGTCGC3400.048.37749549
TCGCCGT3250.047.3863952
GTCGCCG3400.047.3547151
TGTCGGT305.8729725E-446.6684143
Produced by FastQC (version 0.11.5)