FastQCFastQC Report
Wed 25 May 2016
SRR1780981_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780981_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362892
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT4910213.5307474400097No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG55701.534891923767953No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31850.8776715937524111No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC31040.8553509032990532No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT15680.432084476924264RNA PCR Primer, Index 24 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC12650.3485885607839247No Hit
ATTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT7650.21080652094838132No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC7500.206673059753315No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC4110.11325683674481664RNA PCR Primer, Index 24 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGACGT3880.10691886291238165No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG3880.10691886291238165No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTA600.070.0049645
GCCGTAT600.064.1712146
AGGGGGG59950.063.39859870
AGCCGTC650.059.23496645
CTTTATA2450.055.7182352
CCGTCTT70900.053.86136247
CGTCTTC71100.053.80831548
GCCGTCT71050.053.79691746
TCTTCTG71300.053.50272850
AAGGGGG70950.053.470769
GTCTTCT71400.053.4351649
CTGCTTG71150.053.2220254
ATGCCGT73600.053.21708344
CTTCTGC71500.053.20622651
TCTCGTA75250.053.02700838
TATGCCG74150.053.01117343
TGCCGTC73250.052.9935245
CTCGTAT75000.052.9704239
CATCTCG75550.052.9554436
ATCTCGT75550.052.9091137