FastQCFastQC Report
Wed 25 May 2016
SRR1780980_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780980_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606420
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8331013.738003364005143No Hit
CTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTG27150.4477095082616009No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAT150.002225196269.993651
CAGTCGT150.002225196269.993651
CGCGTGG207.929164E-569.9936457
GGTCGCT200.00694842252.50389553
GGTACAT200.00695295652.4952351
GCTACCG200.00695295652.4952351
TCGCCGT4950.049.49862352
TCGGTGG4850.049.0717645
CGGTGGT4900.048.5710346
CTCGGTG4900.047.85674744
GTCGCCG5050.047.8253351
GCCGTAT4950.047.37725454
GATCTCG5000.046.89961241
TGGTCGC5000.046.89961249
GGGCGCT305.8774324E-446.66628313
ATCTCGG5050.046.4352642
AGATCTC5150.046.209440
TCTCGGT5800.043.44791443
GTAGATC5750.041.9961938
CGTATCA5800.041.63758556
Produced by FastQC (version 0.11.5)