FastQCFastQC Report
Wed 25 May 2016
SRR1780980_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780980_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences606420
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT566159.33593878829854No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG68121.123313874872201No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45270.7465123181953102No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC28900.47656739553444805No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT17780.2931961346921276TruSeq Adapter, Index 12 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC15040.24801292833349825No Hit
ATTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT9410.15517298242142408No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC9120.15039081824478084TruSeq Adapter, Index 12 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCGT252.8387585E-670.002561
GTCTGCC150.002224801669.9967942
CTGCCGT150.002224801669.9967944
TGCCTCG150.002224801669.9967943
AGGGGGG76750.060.96788470
CAACTTG950.058.9495228
GATCTTA308.373525E-658.335461
GACAACT1150.057.82819726
AGCCGTC750.055.9974345
AGCCGAC252.390676E-455.9974345
GAATCTC509.822543E-1055.9974334
GCCGTCT89050.053.0227146
CCGTCTT88750.052.96531747
CGTCTTC89850.052.94016648
TCTTCTG89750.052.6871650
CAGCGTG200.006949458352.5019151
CTCGTAC200.006949458352.50191546
CGTCTAC608.367351E-1152.50191548
GGACCTA200.006949458352.5019151
TCTGCCG200.006951724652.49758543
Produced by FastQC (version 0.11.5)