FastQCFastQC Report
Wed 25 May 2016
SRR1780979_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780979_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379301
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3924310.346136709368022No Hit
CTTATACACATCTGACGCTGCCGACGATGCACGAAGTGTAGATCTCGGTG7540.1987867155636289No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAC200.006949396352.4982251
TCTATGC200.006949396352.4982251
TTTAGTC200.006953020652.491358
ACCTGGT200.006953020652.491357
GAGACGG200.006953020652.491361
GTCCAGT305.875375E-446.665091
TGGTCGC1900.046.04500249
TCGCCGT1550.045.1597652
CTCGGTG2100.044.99254244
GCCGTAT1650.044.5439454
GGACGCG404.5934492E-543.7427545
GATCTCG2100.043.33757841
TCGGTGG2400.042.2846645
TCTCGGT2500.041.9930443
CCCAACG602.589004E-740.8427221
GTCGCCG1800.040.83194751
CGCAAAG701.9252184E-839.99337412
CGGTGGT2300.039.55866246
CTGTACG459.184941E-538.89269641
ATCTCGG2350.038.7220942
Produced by FastQC (version 0.11.5)