FastQCFastQC Report
Wed 25 May 2016
SRR1780979_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780979_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences379301
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT247176.516460541891532TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG30980.8167655766792074TruSeq Adapter, Index 5 (95% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16140.4255195741640544No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC14290.37674564527907917RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT11660.307407573404763TruSeq Adapter, Index 5 (96% over 26bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC7610.20063221557549282RNA PCR Primer, Index 5 (95% over 21bp)
ATTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT6060.15976757245564868No Hit
CTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC4620.12180300078301928TruSeq Adapter, Index 5 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG33250.058.94659470
AGCCGTC308.369971E-658.33256545
CGAATGC750.056.00664541
ATTCCGT252.3891435E-455.9992644
CCGACTT252.3891435E-455.9992647
ATATCGT700.055.00652337
GCTTAAG200.00694520452.5062339
TCGAAAG200.00694520452.5062340
GGGTCAC200.00694882652.4993116
CCATCCG200.00694882652.4993133
CGTGTCC200.00694882652.4993122
CGCTTGC200.00694882652.4993144
CCGTCTT39200.051.60646447
GCCGTCT39450.051.3681446
TATGCCG41300.051.18576443
CGTCTTC39900.051.13967548
TCTTCTG39150.050.8677850
CGTATGC41650.050.76232541
GTATGCC41700.050.53357342
ATCTCGT40950.050.43333437
Produced by FastQC (version 0.11.5)