FastQCFastQC Report
Wed 25 May 2016
SRR1780978_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780978_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511186
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG423088.27643949560434No Hit
CTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTG63231.2369274588897192No Hit
ATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGGTC6930.13556709299550457No Hit
TCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT6390.12500342341143927No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGCT200.00695368152.4926644
TACCGAT200.00695368152.4926647
GCCGTAT9200.047.56165754
CGGTGGT9400.046.9083446
TCGCCGT9350.046.79863752
GATCTAC305.874702E-446.6692771
TGGTCGC9300.046.6647149
TCGGTGG9450.046.2898345
ATCTCGG9350.046.03634642
GTAGATC9400.045.80043438
CTCGGTG9500.045.67782644
GTCGCCG9550.045.45201551
GATCTCG9500.045.3138941
TAGATCT9600.045.2108639
AGATCTC9550.045.07664540
TCTCGGT9700.044.7360243
CGCCGTA9850.044.42307353
GGTGGTC10500.042.3274247
CGTATCA10450.041.8683756
GTGGTCG10550.041.79510548
Produced by FastQC (version 0.11.5)