FastQCFastQC Report
Wed 25 May 2016
SRR1780978_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780978_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences511186
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT219534.294522932944173No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG100731.970515624449809No Hit
CTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT27110.5303353378222408No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG24880.4867112949102675No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC14070.27524227971814563No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT10270.20090534560805653RNA PCR Primer, Index 48 (95% over 23bp)
CATATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT8620.16862746632341263No Hit
CAAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT6250.12226469426001493No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC5570.10896229552452533No Hit
TCATATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG5310.1038760842433087No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAA150.00222356770.0049930
ACGTATG352.96719E-760.01602640
AGGGGGG39000.058.96574470
AACTCGT452.6939233E-854.45898437
ATATCCG452.7002898E-854.44299710
TCGACCA200.006942504652.5140240
CGTCTTC42550.052.40091348
CCGTCTT42300.052.04862647
TATGCCG43050.051.8736243
GCCGTCT42550.051.82507746
CGTATGC43650.051.64171241
ATCTCGT43350.051.6054737
TCGTATG44150.051.54263740
TCTTCTG43400.051.52585250
AATCTCG43400.051.38468636
ATGCCGT43200.051.28837644
CTCGTAT43900.051.27792739
TCTCGTA43700.051.2722738
GTCTTCT43850.051.24652549
TGCCGTC43300.051.1699345