FastQCFastQC Report
Wed 25 May 2016
SRR1780977_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780977_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311223
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4555114.636129077863783No Hit
CTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTG81552.6203076250791235No Hit
TCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT8390.2695816183251238No Hit
ATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGGTC8390.2695816183251238No Hit
CTCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG5890.18925336495053388No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTCGA150.002221513870.01478623
CGCGTCT150.002224347869.992297
TCCAACG252.3896199E-455.9938345
CGAAGCT200.006941496452.5110921
CCATGGT200.0069459152.5026539
TGACCGG200.0069459152.5026535
CGCCGTC200.0069459152.5026531
GCGCGTC200.00695032652.4942176
GAACGAC200.00695032652.49421747
GTCCAAC200.00695032652.49421744
ACCTTAT200.00695032652.4942177
TCGCCGT13450.047.8834652
GCCGTAT13550.047.78838754
GTCGCCG13500.047.70611251
TGGTCGC13500.047.70611249
ATCTCGG13750.047.59475742
CTCGGTG13750.047.59475744
TCGGTGG13750.047.59475745
CGGTGGT13850.047.5037946
AGATCTC13750.047.34784740
Produced by FastQC (version 0.11.5)