FastQCFastQC Report
Wed 25 May 2016
SRR1780977_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780977_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311223
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT228737.349392557747982No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG130934.206951285734024No Hit
CTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT32261.0365557815457083No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG29310.9417684425636922No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC18860.6059963434579063No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT10530.33834260321377274TruSeq Adapter, Index 2 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT9690.31135231007991054No Hit
CAAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT7060.22684698752984198No Hit
TCATATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG6660.21399446698990754No Hit
ATTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT5980.1921451820720191No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC4750.15262368141172086No Hit
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC4020.12916783142634058No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACGA150.002223375770.0000170
CGTTGGA150.002223375770.0000113
TGACGTA150.002223375770.0000145
CGCGACG150.002223375770.0000169
AGGGGGG39350.062.08386270
TCACCGA1050.060.00000411
CGTCTAC700.060.048
CGTAACT650.059.23076634
TGACGAC308.365798E-658.33333645
GACGACT308.365798E-658.33333646
AGAGACG601.8189894E-1258.33333623
AAAAGCG252.3883188E-456.00000467
AGACGAT252.3883188E-456.00000425
TAACTCG750.056.036
TGCCGAC750.056.045
AATCTCG950.055.2631636
GCCGACT700.055.046
ACGTAAC700.055.033
CACCGAG1400.055.012
AACGTAT608.367351E-1152.50000432
Produced by FastQC (version 0.11.5)