FastQCFastQC Report
Wed 25 May 2016
SRR1780976_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780976_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310848
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3249510.453662240065885No Hit
CTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTG46041.4811097385217213No Hit
ATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGGTC6380.20524500720609432No Hit
CTCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG4180.1344708667901997No Hit
TCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT4040.1299670578546428No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATG150.002221021870.0186736
GTTACAC150.002221021870.0186725
GTCCGAT150.002221021870.0186735
GTTGCTC150.002221021870.0186725
GCAAGTT150.002223859369.9961442
GGTACGG150.002225278969.984881
GGTCCCA207.928009E-569.984881
GGCCACT150.002225278969.984881
GATCTTA308.376492E-658.3207361
ACAGGTA252.390865E-455.9879042
GTACATC200.006939962552.51400436
GATTGCA200.006939962552.51400436
TGGTCCG200.006939962552.51400433
CTATCAG200.006944381652.50555419
AAGTACG200.006953226452.4886658
GCACCTC200.006953226452.488661
GTTCATG200.006953226452.488661
CAAGATC200.006953226452.4886665
GGTTACA200.006953226452.488661
CTCGGTG6950.047.8390944
Produced by FastQC (version 0.11.5)