FastQCFastQC Report
Wed 25 May 2016
SRR1780976_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780976_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310848
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT160915.176484970146181No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG79242.5491558575252213No Hit
CTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT21590.6945516779905291No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG19560.6292464484249537No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC14150.45520640312950383No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT9660.3107628165534281RNA PCR Primer, Index 47 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT7170.23065935762816553No Hit
CAAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT4960.15956351657401688No Hit
ATTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT4160.1338274655136916No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC4150.1335057648754375No Hit
TCATATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG3920.12610665019559397No Hit
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC3220.10358760551780935No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCCAT150.002222420670.007561
CGTCTAC150.002223839569.996348
AAGCCGT301.0221993E-769.996344
ATCGCCA150.002223839569.99639
AACTCGT453.8380676E-1062.2389637
AGGGGGG28900.058.8739670
GCCTTTA308.360417E-658.3396341
GTCCTCG200.00693990452.5141234
TCGCGCG200.00693990452.5141238
CAGGTAT200.00694432252.5056721
AAGCGTC200.00694432252.5056721
GGACCTA200.00694432252.5056721
AGCGTAA200.006948742552.49722363
GGTCCTC200.006948742552.49722333
CGTCTTA200.006948742552.49722348
TTCAAGG200.006948742552.49722362
TTAAGCA200.006948742552.49722350
ATGACGA200.006948742552.49722344
GACGACT200.006948742552.49722346
GTAACAA200.006948742552.49722333
Produced by FastQC (version 0.11.5)