FastQCFastQC Report
Wed 25 May 2016
SRR1780975_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780975_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401133
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4130710.297582098705417No Hit
CTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTG70171.7492951215681583No Hit
ATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGGTC8170.20367309595570546No Hit
TCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT7270.18123664719681504No Hit
CTCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG5540.13810880680472562No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGTC308.375317E-658.3273144
TGCCGCG200.0069447452.5076723
GTGGACG200.006951592452.4945848
GCCGTAT12050.049.08206654
TCGCCGT11900.048.8184952
GTCGCCG12100.048.59002351
TCGGTGG12550.048.24202745
TGGTCGC12150.048.10202849
ATCTCGG12450.048.0793142
CGGTGGT12350.047.8897946
CTCGGTG12500.047.59508544
AGATCTC12600.047.50693540
GATCTCG12600.047.50693541
GTAGATC12650.047.04244238
TCTCGGT12850.046.8492543
CGCCGTA12550.046.84774853
TAGATCT12750.046.6734839
TTCTTGA305.8777933E-446.66184616
CGTATCA12750.045.838456
GTGGTCG12950.045.40071548
Produced by FastQC (version 0.11.5)