FastQCFastQC Report
Wed 25 May 2016
SRR1780975_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780975_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401133
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT215455.371036539003273No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG114622.857406396382247No Hit
CTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT28580.712481895032321No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG26250.6543964221343046No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC16040.39986737565844743No Hit
CATATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT8460.21090261833357016No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT8270.20616603470669329Illumina PCR Primer Index 9 (95% over 22bp)
CAAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT6200.15456220256124525No Hit
TCATATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG6010.1498256189343684No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC4890.12190470492330474No Hit
ATTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT4660.11617094579603274No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAT207.922755E-569.9992539
TAACGTA150.0022239469.9992538
CGTAATC207.922755E-569.9992548
TCGAAAG150.0022239469.9992540
AGGGGGG37950.058.8399570
CGTACCT200.006949048552.4994452
ACAGACT200.006949048552.4994410
CAATACG200.006949048552.4994469
ACGGTGG200.006949048552.4994415
CCGTCTT41250.052.2661147
CGTCTTC41750.052.22698648
TATGCCG42200.052.16768643
GCCGTCT41400.052.16127846
TCTTCTG41550.052.1414450
CGTATGC42700.051.8846941
ATCTCGT42200.051.83593837
TCGTATG43100.051.7279840
CTCGTAT42450.051.6955639
GATCTCG43000.051.68549336
TCTCGTA42550.051.6563238