FastQCFastQC Report
Wed 25 May 2016
SRR1780974_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780974_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169399
Sequences flagged as poor quality0
Sequence length76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4260425.150089433821925No Hit
CTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTG84835.007703705452807No Hit
TCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT9380.5537222769910094No Hit
ATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGGTC8570.5059061741804851No Hit
CTCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG5590.3299901416183095No Hit
TCTTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG3530.2083837566927786No Hit
CTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGG1710.100945105933329No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGCGT150.002222475869.9932121
AAAAAGG8900.054.28042670
GTACTAG200.006936413252.5104127
TCTTGCG200.006944521352.4949072
ACTCTCG200.006944521352.49490713
ATATCAG200.006944521352.4949071
GTTGTGA200.006944521352.4949071
ACCGTCG200.006944521352.49490743
TTTTGGC200.006944521352.49490745
CCGCAAA200.006944521352.49490752
GGTAGTC200.006944521352.4949077
TACGTGG200.006944521352.49490720
CGGACTG200.006944521352.4949075
CGTATCA12600.046.1066456
GCCGTAT12600.046.1066454
ATCATTA12850.046.0266659
CGCCGTA12550.046.0114753
CCGTATC12650.045.924455
TCGCCGT12600.045.82888852
CGGTGGT12950.045.67124646