FastQCFastQC Report
Wed 25 May 2016
SRR1780974_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780974_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169399
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT2299513.574460297876612No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG132037.794024758115456No Hit
CTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT29821.760340970135597No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG27691.6346023294116256No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC14070.8305834154865142No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT9300.5489996989356489RNA PCR Primer, Index 42 (95% over 22bp)
CATATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT8230.48583521744520336No Hit
CAAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT6260.36954173283195296No Hit
TCATATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG5860.3459288425551509No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC5690.33589336418751No Hit
ATTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT5210.30755789585534743No Hit
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC4180.24675470339258201RNA PCR Primer, Index 32 (95% over 21bp)
ATAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT1910.11275155107173006No Hit
CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC1760.10389671721792927No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACT550.063.63448346
AGGGGGG35200.062.83905470
CGTCTAC453.8198777E-1062.22038748
ATCTCGA401.060107E-861.2481937
TCGAATG401.060107E-861.2481940
CTAATAC4450.060.560011
ATCGATC352.961333E-759.9982333
AATCGAT352.961333E-759.9982332
GCCCTAG308.350866E-658.331611
CGAATGC308.350866E-658.3316141
CGTAAGC553.6379788E-1157.27103841
AATACAC7050.056.11423
TATAGGT252.3820685E-456.0148854
TCAATCG509.749783E-1055.99834430
TCGTAAG509.749783E-1055.99834440
CACGTAT252.3855483E-455.99834439
CAATCGA509.749783E-1055.99834431
TAATACA5400.055.7555542
AAGGGGG40350.054.55848369
CTCGAAT452.6859198E-854.4428439