FastQCFastQC Report
Wed 25 May 2016
SRR1780973_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780973_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117335
Sequences flagged as poor quality0
Sequence length76
%GC76

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6119852.15664550219458No Hit
CTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTG108289.228278007414668No Hit
TCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT11751.0014062300251416No Hit
ATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGGTC11700.9971449269186518No Hit
CTCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG8520.72612604934589No Hit
TCTTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG4310.36732432777943497No Hit
CTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGG1840.1568159543188307No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1400.11931648698171901No Hit
CTTTACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGG1360.11590744449652703No Hit
CTTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT1280.1090893595261431No Hit
CTTATACACATCTGACGCTGCCGACGAATGATGGAGTGTAGATCTCGGTG1200.10227127455575914No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTAAA150.00221792870.0157833
GCAACAC150.00221792870.0157827
ACGACGC150.00221792870.0157832
CCTTATA207.906888E-569.985931
GGGGAAC150.002221683769.9859349
AGCTTAA150.002221683769.9859344
CCTACAT150.002221683769.9859317
GTTGTGC150.002221683769.985931
AGCGCTT150.002221683769.9859310
CCGAATC150.002221683769.9859355
AAAGCAG150.002221683769.9859316
TCTTTAT600.064.153771
AAAAAGG10100.054.7881870
CAAGAAT200.006918689752.53423366
ACACGAC200.00693037752.5118330
GATCGAG200.00693037752.5118330
GCAAAGC200.00693037752.5118322
CACGACG200.00693037752.5118331
AAGCAAC200.00693037752.5118325
CGGGCTC200.00693037752.5118342
Produced by FastQC (version 0.11.5)