FastQCFastQC Report
Wed 25 May 2016
SRR1780973_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780973_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117335
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT3217227.418928708399026No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1725914.70916606298206No Hit
CTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT41103.5027911535347513No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG38063.243703924660161No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC24332.0735500916180167No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT15711.338901436059147RNA PCR Primer, Index 15 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT11981.0210082243149956No Hit
CAAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT9340.7960114202923254No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC8140.6937401457365662No Hit
TCATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG7950.6775471939319043No Hit
ATTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT6060.5164699365065837No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC5880.5011292453232199No Hit
CTTAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT2530.2156219371883922No Hit
CTTTACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC2460.20965611283930624No Hit
TATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCT2400.20454254911151828No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGATAATCTCGTATGCCGT2220.18920185792815442No Hit
CTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG1960.1670430817744066No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC1920.16363403928921463No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTATCTCGTATGCCGTC1900.16192951804661865No Hit
TTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC1890.16107725742532067No Hit
CTCATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC1840.1568159543188307No Hit
ATAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT1830.1559636936975327No Hit
CTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG1800.15340691183363872No Hit
TCTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG1780.15170239059104274No Hit
CTAAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT1780.15170239059104274No Hit
TCAAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG1740.14829334810585076No Hit
CCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG1340.11420292325393104No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTAAATCTCGTATGCCGT1280.1090893595261431No Hit
TCATTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC1200.10227127455575914No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGGA150.002219913370.0000166
AACAATT150.002219913370.0000137
CGGAATA150.002219913370.0000169
GCTATTA150.002219913370.0000125
CCTACCA150.002219913370.0000157
CTTATAA150.002219913370.000011
TTCGGAA150.002219913370.0000167
ACTCCAC150.002219913370.0000167
CGACTAA150.002219913370.0000148
TGATAGA353.6743586E-970.055
AACCGAG207.8990204E-570.012
AGCCGAC252.8251106E-670.045
GAGACTT207.8990204E-570.024
ATATCCG353.6743586E-970.010
CGAGACT207.8990204E-570.023
ACGTATG207.8990204E-570.040
TCATAAT207.8990204E-570.01
CTGATAG353.6743586E-970.054
TATCCGA353.6743586E-970.011
CTAATAC5950.065.8823551
Produced by FastQC (version 0.11.5)