FastQCFastQC Report
Wed 25 May 2016
SRR1780972_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780972_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146766
Sequences flagged as poor quality0
Sequence length76
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5726639.018573784118935No Hit
CTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTG91926.263030947222109No Hit
ATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGGTC9820.669092296580952No Hit
TCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT9290.6329803905536705No Hit
CTCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG6640.45242086041726287No Hit
TCTTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG3350.22825450036111908No Hit
CTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGG1530.1042475777768693No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCAT150.0022167470.03409656
TGCGCCC150.002219740570.0102319
ACGTGCA150.002219740570.0102330
ACTCGCA150.002219740570.0102355
TGCACGT150.002219740570.0102327
CGTATTA150.002222743869.98637449
AACGGAG150.002222743869.98637411
TACGCGT150.002222743869.9863745
TATTCAG150.002222743869.98637467
TCCGTAT150.002222743869.98637447
CGTGTGG150.002222743869.9863749
TCTGGCG252.8319282E-669.9863711
TGCCGGC453.8016879E-1062.2313119
CGCGTCT308.353505E-658.3219767
ACTGCAC252.3824244E-456.00817525
TGACCGC252.3824244E-456.00817550
AGTACAC252.3824244E-456.00817535
ATGTAAG252.3864416E-455.98909415
AAACTGC200.00693600852.50766823
GACCGCG200.00693600852.50766851
Produced by FastQC (version 0.11.5)