FastQCFastQC Report
Wed 25 May 2016
SRR1780972_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780972_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146766
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT3068720.908793589796No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG146259.964841993377213No Hit
CTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT37702.5687148249594594No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG36782.506030006949839No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC19941.3586252946867803No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT12900.8789501655696823Illumina PCR Primer Index 9 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT12490.8510145401523515No Hit
CAAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT8440.5750650695665209No Hit
TCATATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG7690.52396331575433No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC7200.49057683659703205No Hit
ATTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT6110.41630895438998133No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC5050.34408514233541826No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT2260.15398661815406836No Hit
CTAAAACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT2120.1444476241091261No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC2100.14308491067413434No Hit
TCTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG2040.13899677036915906No Hit
ATAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT1870.12741370617172915No Hit
TCAAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG1820.12400692258424977No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC1800.12264420914925801No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG1760.1199187822792745No Hit
TATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCT1700.11583064197429924No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG1500.10220350762438167No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGCTCTCGTATGCCGT1500.10220350762438167No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGACG150.002219098270.01533543
TCGTATC150.002219098270.01533540
GTATCCC150.002219098270.01533542
CTTACAT150.002219098270.01533527
CGAGAAC252.8306986E-669.99148615
CTGCTTA207.910395E-569.9914854
GCAGTTT150.002222100569.9914819
ATCAACG207.910395E-569.9914810
CTGCGCA150.002222100569.9914811
TGCGCAA150.002222100569.9914812
ACGTGCA150.002222100569.9914815
TCTCGAA750.065.3476538
AGGGGGG51250.064.87015570
CGAATGC550.063.6503141
GCACACG550.063.6286218
CTCGAAT750.060.6799639
TCGAATG750.060.6799640
TCTTTAT1100.060.4884071
CTTTATA1100.060.4884072
ACTTGCA352.953493E-760.0131527
Produced by FastQC (version 0.11.5)