FastQCFastQC Report
Wed 25 May 2016
SRR1780971_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780971_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411135
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4626111.252021841974047No Hit
CTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTG72021.751736047770197No Hit
ATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGGTC7200.17512495895508773No Hit
TCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT7010.17050360587155072No Hit
CTCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG4330.10531820448271248No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG200.00695136152.4952551
TCTAATG200.00695470652.4888760
GCCGTAT11650.046.26754454
TCGCCGT11800.045.38277452
CTCGGTG11900.045.29553244
GATCTCG12000.045.2152541
TCGGTGG12000.045.20974745
CGGTGGT11850.045.1857946
ATCTCGG12100.045.1308742
AGATCTC12100.044.8415740
GTAGATC12100.044.8415738
TCTCGGT12100.044.5522743
TGGTCGC11950.044.5148149
GTCGCCG12050.044.44122351
GTCTACT404.592081E-543.7460441
CGTATCA12200.043.60261556
CGCCGTA12300.043.53794553
TAGATCT12500.043.40663539
GTATCAT12500.041.99109657
GTGGTCG12750.041.71664448
Produced by FastQC (version 0.11.5)