FastQCFastQC Report
Wed 25 May 2016
SRR1780971_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780971_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411135
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT232905.664805963977769No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG115022.7976212193075267No Hit
CTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT28520.6936894207498754No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG27120.6596373453974971No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC14320.3483040850328968No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT8770.21331192917168326RNA PCR Primer, Index 30 (95% over 22bp)
CATATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT8220.19993432814039186No Hit
CAAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT5700.13864059250611113No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC5660.13766767606747174No Hit
TCATATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG5400.1313437192163158No Hit
ATTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT4780.11626351441740548No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAAGC150.00222345670.003437
AGGGGGG39000.060.75197270
GCCGTAT900.054.44709846
CGTCTTC40150.054.3114848
CCGTCTT40450.054.25480347
GCCGTCT40800.053.96095746
TATGCCG43200.053.63686843
CGTATGC43050.053.4985441
TCTTCTG39200.053.4782450
ATGCCGT43250.053.25114444
TCTCGTA45150.053.10335538
TGCCGTC42500.053.03787645
GTCTTCT39400.053.03557649
TCGTATG44500.052.93515840
ATCTCGT45350.052.86916437
CTTGAAA42700.052.78945557
CTCGTAT44600.052.7379939
GTATGCC44250.052.6805342
GATCTCG45950.052.63585736
CTTCTGC39700.052.628451