FastQCFastQC Report
Wed 25 May 2016
SRR1780970_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780970_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125305
Sequences flagged as poor quality0
Sequence length76
%GC72

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5680345.331790431347514No Hit
CTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTG1277210.192729739435777No Hit
TCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT13091.0446510514344998No Hit
CTCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG8770.6998922628785762No Hit
ATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGGTC6970.5562427676469415No Hit
TCTTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG4090.3264035752763258No Hit
CTTATACACATCTGACGCTGCCGACGAATGATGGAGTGTAGATCTCGGTG1450.11571764893659471No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAAC150.002217807870.0195649
GCAGAGT150.002217807870.0195651
CAGAGTT150.002217807870.0195652
GTACGAA150.002217807870.0195655
CTTGTGC207.905808E-569.991621
TCACCTA150.00222132369.9916222
TTGTGCG207.905808E-569.991622
TTTACAC301.0176882E-769.991622
TGGACAA150.00222132369.9916241
TAGCGGC150.00222132369.991627
TCGGGAT150.00222132369.9916221
CGCGCAC150.00222132369.9916210
ATAGAGT150.00222132369.9916231
GCTCTGG207.905808E-569.9916230
ACAGGCA150.00222132369.991623
CTACGCT150.00222132369.9916226
GTCGTAA150.00222132369.9916247
TCGTAAA150.00222132369.9916248
ACGTCAC150.00222132369.9916219
AATAGCG150.00222132369.991625
Produced by FastQC (version 0.11.5)