FastQCFastQC Report
Wed 25 May 2016
SRR1780970_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780970_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125305
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT3098924.730856709628508No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1892315.101552212601252No Hit
CTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT38333.0589361956825347No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG36842.9400263357407925No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC23451.8714337017676868No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT11410.9105781892183072Illumina PCR Primer Index 10 (95% over 23bp)
CATATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT11250.8978093451977176No Hit
CAAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT8640.6895175771118471No Hit
TCATATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG7380.5889629304497027No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC7020.5602330314033758No Hit
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC5150.41099716691273297Illumina PCR Primer Index 10 (95% over 21bp)
ATTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT4960.3958341646382825No Hit
CTTAAACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT2690.21467619009616534No Hit
CTTTACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC2180.17397549978053548Illumina PCR Primer Index 10 (95% over 21bp)
TCTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG1820.14524560073420853No Hit
ATAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT1800.1436494952316348No Hit
TTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC1790.14285144248034795Illumina PCR Primer Index 10 (95% over 21bp)
CTAAAACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT1740.13886117872391365No Hit
CTCATATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC1740.13886117872391365No Hit
TATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCT1700.13566896771876621Illumina PCR Primer Index 10 (95% over 22bp)
CTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG1680.1340728622161925No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGACGT1660.13247675671361878No Hit
CTTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG1550.1236981764494633No Hit
TCAAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG1460.11651570168788156No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC1440.11491959618530785No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGCGG150.002217105470.0251554
CGTGGTG150.002220619569.9972118
AGACTTG150.002220619569.9972126
GACAGTC150.002220619569.9972133
AGCGTAG150.002220619569.997217
TAGGTGA150.002220619569.9972111
GACGTGG150.002220619569.9972116
CCGTGGC150.002220619569.9972113
TTCCGTC150.002220619569.9972145
ATTCCGT150.002220619569.9972144
GAGCGTA150.002220619569.997216
TATTCCG150.002220619569.9972143
ACCAGAG150.002220619569.997212
TCCGTCT150.002220619569.9972146
GGACAGT150.002220619569.9972132
GTGACGT150.002220619569.9972114
CATCCCA150.002220619569.9972146
AGAGCGT150.002220619569.997215
CGTCTTA700.064.99740648
AGGGGGG49100.064.8650370
Produced by FastQC (version 0.11.5)