FastQCFastQC Report
Wed 25 May 2016
SRR1780969_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780969_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283418
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4696016.56916639027867No Hit
CTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTG77252.7256560980601092No Hit
ATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGGTC10010.3531885765900543No Hit
TCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT7040.2483963615578404No Hit
CTCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG6330.2233450239575468No Hit
TCTTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG3350.11819997318448369No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAGCG150.002221502170.0132438
TTCCGGT200.00694146452.5099342
CGGTCTT200.00694146452.5099345
CGCTAAG305.86639E-446.67549536
GCGCTAA305.86639E-446.67549535
AGCGCTA305.8714976E-446.6672634
ACCGTGT305.876609E-446.6590249
CGCCGTA11300.045.53154453
GCCGTAT11400.045.4391754
TCGCCGT11350.045.33096752
CGGTGGT11800.044.788746
GGTGGTC11900.044.6985647
ATCTCGG11800.044.49994342
CTCGGTG11800.044.49994344
TGGTCGC11800.044.48423449
TCTCGGT11900.044.42016643
AGATCTC11850.044.30435640
GTCGCCG11800.044.19547351
GATCTCG11900.044.12599641
CCGTATC11600.044.05227755