FastQCFastQC Report
Wed 25 May 2016
SRR1780969_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780969_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283418
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT253368.939446330155459No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG126304.45631540692546No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG30061.0606242369927106No Hit
CTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT27420.9674756014085203No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC19640.6929693950278387No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT11020.38882498641582397RNA PCR Primer, Index 24 (95% over 23bp)
CATATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT8950.31578798806003855No Hit
CAAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT6190.21840532358565795No Hit
TCATATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG5630.19864652209810243No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC5350.18876712135432472No Hit
ATTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT5170.18241607801903903No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC4350.1534835472694042No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAA207.914967E-570.00600446
TGACGAC207.9218895E-569.99364545
AGGGGGG39750.060.66115670
GACGACT252.3869419E-456.004846
TTTCGCC200.006944305752.504529
CGTAATC200.006944305752.504548
CGACTTA200.006944305752.504548
GGGTCTA200.00694915452.4952351
ATCGTAA200.00694915452.49523539
ACGTATG407.468843E-752.49523540
AAGGGGG46750.051.80278869
CGTCTTC44850.051.19725448
TCTCGTA47700.050.9911838
CCGTCTT44650.050.9562147
CTCGTAT47400.050.94474439
CATCTCG47750.050.93778636
CGTATGC46600.050.9181341
TGCCGAC552.2791937E-950.9044745
ATCTCGT47750.050.79120337
TCGTATG47850.050.75819840