FastQCFastQC Report
Wed 25 May 2016
SRR1780968_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780968_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271113
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5313719.599576560327243No Hit
CTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTG98783.6434992051284887No Hit
ATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGGTC11100.40942337696827524No Hit
TCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT9860.3636859907123598No Hit
CTCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG7950.29323566188268363No Hit
TCTTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG3730.1375810086569069No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTCAC150.002219395270.0291632
AGCTCGA150.00222427469.9904160
GATCTAA150.002225902269.977515
GCGCTCA150.002225902269.97756
TACCTAC200.006934952.52187327
GCTTTGC200.00695010152.49280543
GTGGACG200.00695517452.48312448
TATCTAG200.00695517452.4831245
GTCAGTA352.0826306E-550.00237356
GATAGTC352.0849055E-549.9931552
TCGCCGT14650.049.44714752
GCCGTAT14800.049.41890354
CTCGGTG14750.049.1119144
TCGGTGG14900.049.08723445
GTCGCCG14850.049.0168551
CGCCGTA14850.049.0168553
ATCTCGG14750.048.87465742
TGGTCGC14900.048.84335749
AGATCTC14950.048.7069540
GATCTCG14850.048.5634541
Produced by FastQC (version 0.11.5)