FastQCFastQC Report
Wed 25 May 2016
SRR1780968_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780968_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271113
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT267349.860832936819703No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG156585.775451564476805No Hit
CTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT38501.4200720732683418No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG33061.219417733564971No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC18700.6897492927303376No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT13790.5086439971524789TruSeq Adapter, Index 12 (95% over 23bp)
CATATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT10550.38913663306444174No Hit
CAAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT8620.31794860445644435No Hit
TCATATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG7670.2829078649861866No Hit
ATTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT6820.2515556244075349No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC5820.21467063549147403No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC5250.19364619180931936TruSeq Adapter, Index 12 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGAAA150.00222062770.0193746
AATGGTC150.00222387969.99354620
AGGGGGG47400.061.28126570
AATCACG650.059.23623336
GCCGTAT308.3491905E-658.34947646
AATGCGT252.3866794E-456.005176
TGCCGTA509.767973E-1056.0051745
GCCGTCA1650.053.0449846
GTAGCCG200.006938738352.5145347
TAGAGCA200.006943803352.504844
ATTCGCA200.006943803352.5048439
CTTGACA200.006943803352.5048431
ACGTATG608.367351E-1152.5048440
GCAGCGT200.006943803352.504841
GATCTAC200.006943803352.5048428
CACGTAT608.367351E-1152.5048439
TAAGCGT200.006943803352.504844
TCAAGGG200.006943803352.5048437
TATCGAT200.006943803352.5048442
CTATCGT200.006948871552.4951559
Produced by FastQC (version 0.11.5)