FastQCFastQC Report
Wed 25 May 2016
SRR1780967_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780967_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380364
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5924715.576395242451968No Hit
CTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTG91682.410322743477301No Hit
ATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGTGGTC11610.30523393381077074No Hit
TCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGGT8980.2360896404496745No Hit
CTCTTATACACATCTGACGCTGCCGACGATTGATGGAGTGTAGATCTCGG7920.20822159825851028No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGATA200.00694667352.5034524
GGTACGG200.00695028652.4965481
ACACGGT200.00695028652.4965486
CGGTTCA200.00695028652.4965484
TGGTCGC14150.046.75218649
GCCGTAT13850.046.24852454
CGGTGGT14250.046.172446
TCGGTGG14400.045.9344845
TCGCCGT14100.045.6767652
CTCGGTG14500.045.37632444
GATCTCG14350.045.36883541
GTCGCCG14450.045.297151
AGATCTC14450.045.297140
TCTCGGT14700.044.99704443
GTGGTCG14850.044.77820248
ATCTCGG14500.044.65223342
GTAGATC14750.044.61310238
CGCCGTA14500.044.41671453
CGTATCA14650.044.1950556
TAGATCT14900.044.16397539
Produced by FastQC (version 0.11.5)