FastQCFastQC Report
Wed 25 May 2016
SRR1780967_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780967_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences380364
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT304007.9923441755791815TruSeq Adapter, Index 5 (95% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG150833.9654120789559473No Hit
CTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT38841.0211271308536034TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG36390.9567151465438369TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC20010.526075022872827RNA PCR Primer, Index 5 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT15350.4035607996550673TruSeq Adapter, Index 5 (96% over 26bp)
CATATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT11870.3120694913293582TruSeq Adapter, Index 5 (95% over 23bp)
CAAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT8080.2124280951930256TruSeq Adapter, Index 5 (95% over 23bp)
TCATATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG7140.1877149257027479TruSeq Adapter, Index 5 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC6930.1821938984761965RNA PCR Primer, Index 5 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC5150.13539662007971312TruSeq Adapter, Index 5 (95% over 24bp)
ATTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT4750.12488037774342471No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTA353.7052814E-970.0000145
CTTAAGC150.002223756970.051
AGATCGA150.002223756970.012
TTCGCGG150.002223756970.062
AGGGGGG49750.061.34673370
CGTCTAC401.0646545E-861.2548
ACGTATG1350.059.62962740
GCCGTAT308.369359E-658.33333246
CGTAATC252.3889971E-455.99999648
CACGTAT1400.055.00000439
CGACTTC850.053.5294148
TGACGAC200.0069484852.545
TCGATCC200.0069484852.545
ATCCCGG200.0069484852.548
CGAATGC800.052.541
GACGACT200.0069484852.546
GGCTCTA200.0069484852.51
TCGCGGT200.0069484852.563
AAGGGGG58650.051.560169
TATGCCG58550.051.40904643
Produced by FastQC (version 0.11.5)