FastQCFastQC Report
Wed 25 May 2016
SRR1780966_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780966_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154900
Sequences flagged as poor quality0
Sequence length76
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5237433.811491284699805No Hit
CTTATACACATCTGACGCTGCCGACGACGATACACGTGTAGATCTCGGTG3350.21626856036152356No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCCTT207.889494E-570.0313351
TAAATCG150.002217320770.0313324
CTCTCCG150.002217320770.0313324
ACACGTC150.002223009569.986126
GCGACTC150.002223009569.9861241
CCGTGTG150.002223009569.9861215
GTCTATG150.002223009569.9861214
CGTTAAA150.002223009569.9861217
CTGCCGT150.002223009569.9861212
GGTTATC150.002223009569.9861240
TACCGCG150.002223009569.986128
GCCGTAT601.8189894E-1258.3594454
TGGTCGC800.056.86372449
AGTTAGG252.3868991E-455.98889557
TGTCGAA252.3868991E-455.98889511
CGGTGGT900.054.43364746
GTCGCCG800.052.52349551
GATGTAC200.006928464452.5234951
ATGTACA200.006928464452.5234952
TCATGGC200.006928464452.5234952
Produced by FastQC (version 0.11.5)