FastQCFastQC Report
Wed 25 May 2016
SRR1780966_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780966_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154900
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT3236020.890897353131052No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG59123.8166559070367976No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC26111.6856036152356357No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT17531.131697869593286RNA PCR Primer, Index 48 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC10450.6746287927695287No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5850.3776630083925113No Hit
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC5500.35506778566817304No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT4210.2717882504841833No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGACGT3340.21562298256939963No Hit
CTTATACACATCTCCGAGCCCACGAGACTATCGTCAATCTCGTATGCCGT3080.19883795997417691No Hit
CTTTACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC2970.19173660426081343RNA PCR Primer, Index 48 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCAGT2710.17495158166559072No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC2510.16204002582311167No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG2240.14460942543576502No Hit
TATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCT2190.14138153647514526RNA PCR Primer, Index 48 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC2060.1329890251775339No Hit
CCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG1900.12265978050355068No Hit
CTTATACACATCTCCGAGACCACGAGACTTTCGTCAATCTCGTATGCCGT1720.11103938024531956No Hit
CATATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT1710.11039380245319562No Hit
CTTATACACATCTCCGAGCCAACGAGACTTTCGTCAATCTCGTATGCCGT1680.10845706907682374No Hit
CTTATACACATCTCCGAGCCCACGAGACATTCGTCAATCTCGTATGCCGT1670.10781149128469981No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACTC150.00221703770.0335928
GGTCATA150.002219879570.010981
GAGGACT207.913516E-569.988384
CATGAAC150.00222272569.9883867
TACGTTT150.00222272569.9883834
ACGTATG600.064.19746440
ATTCGTC550.063.66689729
TCGACAA550.063.66689731
AGGGGGG42500.063.15422470
TTTCGAC401.05501385E-861.27939629
CTTTCGA401.05501385E-861.27939628
CACGTAT401.05501385E-861.27939639
CCTTATA401.0577423E-861.259611
ATATCCG800.061.23983410
ACATTCG650.059.25919327
TCGTCAT308.322329E-658.3613331
TCACGTA308.322329E-658.3613338
CCGTATT308.338287E-658.3424847
GCCGTAT308.338287E-658.3424846
TCTTTAT1450.057.9401251
Produced by FastQC (version 0.11.5)